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The translocation or shuttling of Hippo proteins between the nucleus and cytoplasm is a rapid event following cytoskeletal or mechanical cues as well as stimulation with extracellular growth factors. Here we describe an experimental procedure for a simple and fast separation of nuclear and cytoplasmic fractions which maintains protein integrity and integrity of protein-protein complexes, indicating that it should be applicable to many experimental questions.

Original publication




Journal article


Methods Mol Biol

Publication Date





115 - 119


Cytoplasmic compartment, Hippo pathway, Hypotonic buffer, Nuclear compartment, Subcellular fractionation, Cell Fractionation, Cell Line, Cell Nucleus, Cells, Cultured, Cytoplasm, Humans, Protease Inhibitors, Protein-Serine-Threonine Kinases, Signal Transduction