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Recombinant plasmids containing mutant or wild-type adenovirus serotype 2 EIa genes that produce the 12S mRNA alone, the 13S mRNA alone, or both mRNAs were cotransfected into HeLa cells with plasmids containing the viral EIIa or EIII transcription units. The amount of RNA produced from the EIIa and EIII promoters was increased by the products of both the 13S and the 12S RNAs. By measuring the level of specific transcription in nuclei isolated from transfected cells we directly demonstrate that the increased amount of EIIa RNA is due to stimulation of the rate of transcription.

Original publication




Journal article


Proc Natl Acad Sci U S A

Publication Date





4381 - 4385


Adenoviridae, Gene Expression Regulation, HeLa Cells, Humans, Operon, Plasmids, RNA, Messenger, RNA, Viral, Recombination, Genetic, Transcription, Genetic, Transfection, Viral Proteins