Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

© 2011 Elsevier B.V. All rights reserved. Cryopreservation plays a significant role in tissue and organ banking. With the rapid development of tissue engineering, cryopreservation of tissue-engineered products is of great importance to meet the 'ready-to-use' requirement in clinical application and regenerative medicine. Cryopreservation can be achieved by conventional freezing and vitrification methods. Due to complexity and intrinsic differences between isolated cells and tissues, evaluation of the success of cryopreservation for tissues and organs is quite different from that of cells and more difficult. In this article, an overview of the methodology of cryopreservation is presented. Current development in cryopreservation of natural and engineered tissue has been reviewed as well.

Original publication





Book title

Comprehensive Biotechnology, Second Edition

Publication Date





83 - 98