Expansion of adipose tissue-derived stem cell in scaffold-spinner flask culture system
Zhu YX., Liu TQ., Song KD., Ma XH., Cui ZF.
Recent reports indicate that adipose tissue is a novel source of multipotent stem cells, which can be used in cell therapy and tissue engineering. However, traditional cultivation of adipose tissue-derived stem cells (ADSCs) is hard to meet the need of clinical applications. In order to obtain a great number of ADSCs, we seeded ADSCs in collagen/chitosan scaffolds and compared the proliferation of ADSCs in three dimensional (3D) static environment in dishes and 3D dynamic environment in spinner flask. The growth dynamic parameters of ADSCs were examined by CCK-8 kit every other day; variations of glucose and lactic acid concentration were analyzed every day. After 14 days, the cells were observed with scanning electron microscope (SEM). The surface markers, the specific transcription factors and the multi-differentiation potential were also assayed to identify the stemness of expanded cells. The results show that in spinner flask, the cells in scaffolds could be expanded by more than 26 times in 14 days, and they present better morphology and vitality and stronger differentiation ability than the cells cultivated in scaffolds statically. All cells maintain stem cell characteristics after proliferation. Therefore, spinner flask cultivation is an easy-to-use inexpensive system for expanding ADSCs in 3D scaffolds.