Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Aim: To investigate the effects of PCA on cultured neural stem/progenitor cells (NS/PCs) proliferation and basal apoptosis. Methods: NS/PCs obtained from 14-day-old rat embryos were propagated as neurospheres and cultured under normal condition with or without PCA for the indicated time. The cell viability was determined by Cell Counting Kit-8 (CCK-8) test, while cell proliferation was assayed by bromodeoxyuridine ( BrdU) labeling. Results: After 4 days, basal apoptotic cells were detected and PCA significantly reduced the levels of apoptosis of NS/PCs. Treatment with 0.06 mmol·L-1 PCA caused 140.32% ±9.17% increase in cell viability compared with the control. Furthermore, on day 4 and day 7, the reactive oxygen species (ROS) level in NS/PCs was depressed by 43.8% ± 4.7% and 54.5% ± 6.1%, respectively, and caspase-3 activity was decreased by 70.6% ± 4.4% and 62.3% ± 5.5% respectively. Conclusions PCA can increase the cellular viability of NS/PCs and stimulate cell proliferation in a dose-and time-dependent manner.

Type

Journal article

Journal

Chinese Pharmacological Bulletin

Publication Date

01/01/2009

Volume

25