Vitrification of corneal endothelial cells in a monolayer.
Fan W-X., Ma X-H., Liu T-Q., Cui Z-F.
An in vitro model was developed for bovine natural corneal endothelia. The cells were cultured to a confluent monolayer and vitrified using 25% (w/w) 1,2-propanediol-35% (w/w) trehalose as cryoprotective agents. Approximately, 61.3% of the cells were viable using the protocol.