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An in vitro model was developed for bovine natural corneal endothelia. The cells were cultured to a confluent monolayer and vitrified using 25% (w/w) 1,2-propanediol-35% (w/w) trehalose as cryoprotective agents. Approximately, 61.3% of the cells were viable using the protocol.

Original publication

DOI

10.1263/jbb.106.610

Type

Journal article

Journal

J Biosci Bioeng

Publication Date

12/2008

Volume

106

Pages

610 - 613

Keywords

Animals, Cattle, Cells, Cultured, Culture Media, Endothelium, Corneal, Propylene Glycol, Trehalose