Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

The architecture and biomaterial are vital for three-dimensional culture of cells in scaffolds, so collagen-chitosan scaffolds suitable for the proliferation of adipose tissue-derived stem cells (ADSCs) were fabricated in this study. Chitosan was fully mixed with collagen with different volume ratio and cross-linked. The microstructure, pore size, bibulous ability, water content, interval porosity, enzyme degradation and affinity were examined before and after cross-linking. During ADSCs cultured in scaffold, the viability and metabolic rates were measured. After 14 days, the surface markers, specific transcription factors and multi-differentiation potential were assayed to identify the stemness of expanded cells. According to the pore size, bibulous ability, interval porosity, degradation rate and affinity of the scaffold, we chose cross-linked scaffolds of 7:3 material ratio as a better scaffold for ADSCs proliferation, and ADSCs could be expanded by more than 20 times. All expanded cells still maintained stem cell characteristics and pluripotency. So our developed collagen-chitosan scaffolds can promote ADSCs adhesion, expansion, and maintain pluripotency.

Original publication

DOI

10.1007/s10856-008-3636-6

Type

Journal article

Journal

J Mater Sci Mater Med

Publication Date

03/2009

Volume

20

Pages

799 - 808

Keywords

Absorbable Implants, Adipose Tissue, Antigens, CD, Biocompatible Materials, Cell Differentiation, Cell Proliferation, Cells, Cultured, Chitosan, Collagen, Cross-Linking Reagents, Glucose, Humans, Lactic Acid, Materials Testing, Microscopy, Electron, Scanning, Stem Cells, Tissue Scaffolds