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The expansion of hematopoietic stem/progenitor cells (HSPCs) from umbilical cord blood (UCB) with the support of microencapsulated osteoblasts under hypoxia environment was investigated. The expansion of HSPCs was evaluated through the total number of UCB mononuclear cells (MNCs) produced, their repopulating potential with the colony-forming unit assay (CFU-Cs) and CD34(+) phenotypic analysis with flow cytometry. At the end of 7 days of culture, the UCB-MNCs, CFU-Cs and CD34(+) cells had achieved 18.7 +/- 1.6, 11.6 +/- 0.9 and 23.4 +/- 2-fold expansions, respectively, in the test groups. These were significantly different from those in control groups. Microencapsulated osteoblasts under hypoxia conditions had therefore a significant effect on the expansion potential of HSPCs in vitro.

Original publication

DOI

10.1007/s10529-009-9961-0

Type

Journal article

Journal

Biotechnol Lett

Publication Date

07/2009

Volume

31

Pages

923 - 928

Keywords

Antigens, CD34, Cell Culture Techniques, Cell Hypoxia, Cell Proliferation, Fetal Blood, Flow Cytometry, Hematopoietic Stem Cells, Humans, Osteoblasts