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The effect of protocatechuic acid (PCA) from Alpinia oxyphylla and catapol from Rehmannia on the proliferation capacity of human adipose tissue-derived stromal cells (hADSCs) was investigated in vitro. Cell counts showed that treatment of hADSCs with PCA for 48 h increased the cell number in a dose-dependent manner, while no obvious effect of catapol on the proliferation of hADSCs was observed. In addition, the cell number of hADSCs treated by 1.5 mM PCA increased in a time-dependent manner. The flow cytometric analysis of DNA content demonstrated the cell cycle progress from the G0/G1 phase to the S phase. Western blot analysis revealed the elevated expression of cyclin D1 in hADSCs induced by PCA treatment. Cyclin D1-siRNA transfection significantly inhibit the promotion of cell proliferation by PCA. Furthermore, the flow cytometric analysis of the cell surface antigens and the multidifferential potential tests of PCA-treated hADSCs showed that the cells retained their functional characteristics of multipotential mesenchymal progenitors. It is concluded that PCA can effectively up-regulate the proliferation of hADSCs.

Original publication

DOI

10.1007/s11010-009-0099-0

Type

Journal article

Journal

Mol Cell Biochem

Publication Date

10/2009

Volume

330

Pages

47 - 53

Keywords

Adipose Tissue, Alpinia, Cell Cycle, Cell Proliferation, Cells, Cultured, Cyclin D1, Dose-Response Relationship, Drug, Humans, Hydroxybenzoates, Kinetics, Mesenchymal Stem Cells, Multipotent Stem Cells, Quaternary Ammonium Compounds, Rehmannia, Stromal Cells