Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

In this paper the influence of NaCl concentration in the binding buffer on the analysis of lysozyme in chicken egg white using MonoQ® anion exchange column is examined. When using a mobile phase having pH 9.5, lysozyme (being positively charged at that pH) is not expected to bind to the anion exchange column. However, when the NaCl concentration in the binding buffer is lower than 70 mM, lysozyme is found to bind to the column, probably due to non- specific adsorption. This results in lysozyme splitting into two peaks; a primary peak in the column void volume, and a secondary lysozyme peak in the NaCl salt gradient. The proportion of lysozyme in the secondary peak decreases with an increase in NaCl concentration in the binding buffer. No secondary peaks are observed when the NaCl concentration in binding buffer is greater than 70 mM. This observation is confirmed by experiments carried out with pure lysozyme.

Original publication




Journal article


Journal of Liquid Chromatography and Related Technologies

Publication Date





1619 - 1626