Effects of cryoprotective agents on the bovine articular chondrocyte viability
Xu X., Cui ZF.
Cryopreservation is the process of choice for long term preservation of cells and tissues. The effects of cryoprotective agents, dimethyl sulfoxide (DMSO), glycerol and 1, 2-propanediol on the bovine articular chondrocyte viability were examined experimentally. The CPA was added at the concentrations of 0.6, 0.9 1.2 and 1.5 mol/L and at 4°C and removed at 37°C was also tested as an alternative protocol. Cell volume excursion during DMSO addition and removal was estimated and correlated well with cell survival rates. Solution makeup affects cell survival rate and a stepwise protocol can improve the cell survival rates significantly.