Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Cryopreservation is the process of choice for long term preservation of cells and tissues. The effects of cryoprotective agents, dimethyl sulfoxide (DMSO), glycerol and 1, 2-propanediol on the bovine articular chondrocyte viability were examined experimentally. The CPA was added at the concentrations of 0.6, 0.9 1.2 and 1.5 mol/L and at 4°C and removed at 37°C was also tested as an alternative protocol. Cell volume excursion during DMSO addition and removal was estimated and correlated well with cell survival rates. Solution makeup affects cell survival rate and a stepwise protocol can improve the cell survival rates significantly.

Type

Journal article

Journal

Chemical Research in Chinese Universities

Publication Date

01/05/2002

Volume

18

Pages

109 - 112