Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

In order to decrease the osmotic damage and chemical cytotoxicity of the cryoprotectant with high concentration, an appropriate scheme of loading and removing the cryoprotective agents is necessary during optimizing the procedure of effective cell and tissue cryopreservation. In this study, the membrane permeability of SD rat cranium parietal bone-derived osteoblasts was studied by microscopy image analysis method. It was found that the osteoblasts isosmotic cell volume V o of the osteoblast is 2585.22 μm 3 , and the osmotic behavior of the osteoblasts behaves as ideal osmometers. According to the Boyle van't Hoff plot, the osmotically inactive volume V b of the osteoblast is 0.335 V o . The found hydraulic conductivities of the osteoblast cell membrane in the presence of cryoprotective agents, dimethyl sulfoxide and 1, 2-propanediol, are 1.5×10 -13 and 5×10 -13 m 3 · N -1 · s -1 , respectively, and the permeability of the above cryoprotectants are 7.5×10 -11 and 120×10 -11 mol · N -1 · s -1 , respectively. The experimental data indicate that the anisotonic condition tolerance of the adherent osteoblasts is lower than that of the osteoblasts in suspension. The study results obtained provide initial biophysical data to characterize osteoblasts and will provide a basis for designing the optimal cryopreservation procedures for engineered bone tissue.

Type

Journal article

Journal

Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities

Publication Date

01/12/2011

Volume

25

Pages

1003 - 1009