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The coculture of cord blood mononuclear cells (CB MNCs) and alginate beads containing rabbit bone marrow (BM) mesenchymal stem cells (MSCs) was investigated in three kinds of culture mediums under the static condition for 7 days. The total nucleated cell density was measured every 24 hours. Flow cytometric assay for CD34+ cells and methylcellulose colony assays were carried out at 0, 72 and 168 h. The results show that the total nucleated cell number is multipled by (15±2.85) times, CD34+ cells (5.33±0.32) times and colony-forming units in culture (CFU-Cs) (5.6±1.21) times respectively at the end of the 7th day's coculture in the serum-free medium supplemented with conventional dose of cytokines. Microcapsules may be the appropriate coculture protocol and the encapsulated rabbit BM MSCs illustrate remarkable effects on CB hematopoietic stem/progenitor cells expansion when serum or proper cytokines are added under the static condition.

Type

Journal article

Journal

Dalian Ligong Daxue Xuebao/Journal of Dalian University of Technology

Publication Date

01/03/2010

Volume

50

Pages

176 - 182