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The feasibility of co-culturing hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs) derived from human umbilical cord blood (UCB) using cytodex-3 microcarriers is investigated in order to assess it as a possibility for future clinical therapies. Considering the safety requirements of clinical applications, human autologous serum (HAS) collected from UCB is used to replace the widely-used fetal bovine serum (FBS). Following to this, after UCB-HSCs and UCB-MSCs ex vivo culture, both can be harvested simultaneously. Besides, after their co-culture, the two different types of stem cells can be easily separated by sedimentation due to the density differences between cytodex-3 microcarriers (containing adherent MSCs) and the suspended HSCs. In order to optimize the culture conditions, the effect of the content of HAS (2.8%, 5.6%, 8.3% and 11.1%) on the expansion of UCB-MSCs and UCB-HSCs is assessed. The results indicate that the expansion of UCB-HSCs is at least (1.88±0.33) fold while supplied with 5.6% HAS, better than that in the other groups, while it has little effect on the expansion of UCB-MSCs. It is concluded that the optimal content of human autologous serum for the co-culture conditions herein reported is about 5.6%. Finally, the co-culture system developed and herein reported is feasible to provide expanded UCB-HSCs and UCB-MSCs for clinical applications.

Type

Journal article

Journal

Dalian Ligong Daxue Xuebao/Journal of Dalian University of Technology

Publication Date

01/09/2012

Volume

52

Pages

631 - 640