Applications of RNA interference high-throughput screening technology in cancer biology and virology.
Gao S., Yang C., Jiang S., Xu X-N., Lu X., He Y-W., Cheung A., Wang H.
RNA interference (RNAi) is an ancient intra-cellular mechanism that regulates gene expression and cell function. Large-scale gene silencing using RNAi high-throughput screening (HTS) has opened an exciting frontier to systematically study gene function in mammalian cells. This approach enables researchers to identify gene function in a given biological context and will provide considerable novel insight. Here, we review RNAi HTS strategies and applications using case studies in cancer biology and virology.