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Planarian adult stem cells (pASCs) or neoblasts represent an ideal system to study the evolution of stem cells and pluripotency as they underpin an unrivaled capacity for regeneration. We wish to understand the control of differentiation and pluripotency in pASCs and to understand how conserved, convergent or divergent these mechanisms are across the Bilateria. Here we show the planarian methyl-CpG Binding Domain 2/3 (mbd2/3) gene is required for pASC differentiation during regeneration and tissue homeostasis. The genome does not have detectable levels of 5-methylcytosine (5(m)C) and we find no role for a potential DNA methylase. We conclude that MBD proteins may have had an ancient role in broadly controlling animal stem cell pluripotency, but that DNA methylation is not involved in planarian stem cell differentiation.

Original publication

DOI

10.1016/j.ydbio.2013.09.020

Type

Journal article

Journal

Dev Biol

Publication Date

01/12/2013

Volume

384

Pages

141 - 153

Keywords

Differentiation, Methyl binding domain, Pluripotency, Regeneration, 5-Methylcytosine, Animals, Cell Differentiation, DNA Methylation, Planarians, Pluripotent Stem Cells