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The paired box homeotic gene 3 (PAX3) is a crucial regulator for the maintenance of melanocytic progenitor cells and has a poorly defined role in melanoma. To understand how PAX3 affects melanocyte and melanoma proliferation, we identified potential PAX3 downstream targets through gene expression profiling. Here, we identify T-box 2 (TBX2), a key developmental regulator of cell identity and an antisenescence factor in melanoma, as a directly regulated PAX3 target. We also found that TBX2 is involved in the survival of melanoma cells and is overexpressed in some melanoma specimens. The identification of TBX2 as a target for PAX3 provides a key insight into how PAX3 may contribute to melanoma evolution and may provide opportunities for prosenescence therapeutic intervention aimed at disrupting the ability of PAX3 to regulate TBX2.

Original publication

DOI

10.1111/pcmr.12029

Type

Journal article

Journal

Pigment Cell Melanoma Res

Publication Date

01/2013

Volume

26

Pages

67 - 77

Keywords

Animals, Cell Lineage, Cell Proliferation, Cell Survival, Chromatin Immunoprecipitation, Gene Expression Regulation, Gene Expression Regulation, Neoplastic, Humans, Melanocytes, Melanoma, Mice, Oligonucleotide Array Sequence Analysis, PAX3 Transcription Factor, Paired Box Transcription Factors, Promoter Regions, Genetic, Protein Binding, T-Box Domain Proteins, Up-Regulation