Effect of neural stem cells on apoptosis of PC12 cells induced by serum deprivation
Li XQ., Liu TQ., Zhu LL., Ge D., Cui ZF.
The apoptosis of pheochromocytoma cells line 12 (PC12 cells) induced by serum deprivation is similar to that of dopaminergic neuron cells. In this paper, the potential cytoprotective effects of neural stem cells (NSCs) on the apoptosis of PC12 cells induced by serum deprivation were investigated to provide experimental and theoretical basis for the therapies of nervous system diseases via NSCs transplantation. The normal PC12 cells were co-cultured with NSCs in DMEM/F12 medium free of serum and then PC12 cell morphologies, viabilities and survival were observed with inverted microscope and assessed with CCK-8 assay, respectively. Besides, the concentrations of glial derived neurotrophic factor (GDNF) in different media were detected with GDNF Elisa kit. Finally, the effect of NSCs on PC12 cell apoptosis induced by serum deprivation and the NSCs' ability to secrete GDNF after co-culture were analyzed. The results show that: (1) PC12 cell apoptosis induced by serum deprivation increases with time and after 72h, only about 44.25% PC12 cells survive; (2) NSCs culture medium protects against PC12 cell apoptosis insignificantly; (3) NSCs' supernatant and NSCs prevent PC12 cells from apoptosis mildly; (4) The amount of GDNF secreted by NSCs increases after co-culture the NSCs with the apoptotic PC12 cells induced by serum deprivation.