Cell carrier function of hollow-fiber membrane in rotating wall vessel bioreactor
Song KD., Liu TQ., Zhao H., Li XQ., Cui ZF., Ma XH.
Large-scale expansion of SD (Sprague-Dawley) rat's osteoblasts was studied in rotating wall hollow-fiber membrane bioreactor (RWHMB) by using hollow-fiber membrane as the carrier. For the sake of contrast, cells were also expanded in T-flask using hollow-fiber membrane as carrier and in rotating wall vessel bioreactor (RWVB) with microcarrier as carrier. During the culture period, the cells were sampled every 12 hr, and after 5 days, the cells were harvested and evaluated with scanning electron microscope (SEM), hematoxylin-eosin (HE) staining and alkaline phosphatase (ALP) staining. Moreover, vcn-Kossa staining and Alizarin Red S staining were carried out for mineralized nodules formation. The results show that in RWHMB, the cells present better morphology and vitality and secrete much more extracellular matrix. It was concluded that the RWHMB combines the advantages of rotating wall vessel and hollow-fiber membrane bioreactors. The hydrodynamic stimulation within it accelerates the metabolism of osteoblast and mass transfer, which is propitious to the cell differentiation and proliferation.