Cookies on this website
We use cookies to ensure that we give you the best experience on our website. If you click 'Continue' we'll assume that you are happy to receive all cookies and you won't see this message again. Click 'Find out more' for information on how to change your cookie settings.

Apoptosis of neurons plays fundamental roles in brain development and disease. Although neurons share with other cell types components of the mitochondrial apoptotic machinery, how this machinery is specifically activated in neurons remains poorly understood. Remarkably, phosphorylation of the BH3-only protein BIMEL at Ser65 triggers apoptosis in neurons but suppresses cell death in non-neural cells. Here, we report that the prolyl isomerase Pin1 interacts with Ser65-phosphorylated BIMEL in neurons. Pin1 is enriched at the mitochondrial membrane in neurons, where it forms a physical complex with the neuron-specific JNK scaffold protein JIP3. Activation of JNK signaling induces the dissociation of Pin1 from JIP3 and concomitantly promotes Pin1 binding to phosphorylated BIMEL. The interaction of Pin1 with phosphorylated BIMEL stabilizes BIMEL and thereby activates neuronal apoptosis. These findings define a neural-specific mechanism of cell death whereby Pin1 couples phosphorylation of BH3-only proteins to activation of the mitochondrial apoptotic machinery.

Original publication




Journal article



Publication Date





655 - 662


Adaptor Proteins, Signal Transducing, Analysis of Variance, Animals, Animals, Newborn, Apoptosis, Apoptosis Regulatory Proteins, Bcl-2-Like Protein 11, Blotting, Western, Cell Count, Cells, Cultured, Cerebellum, Cerebral Cortex, Chaperonin 60, Culture Media, Conditioned, Dose-Response Relationship, Drug, Fluorescent Antibody Technique, Gene Expression, Humans, In Vitro Techniques, Membrane Proteins, Mitochondria, Models, Biological, Mutagenesis, NIMA-Interacting Peptidylprolyl Isomerase, Nerve Tissue Proteins, Neurons, Oligoribonucleotides, Antisense, Peptidylprolyl Isomerase, Potassium Chloride, Proto-Oncogene Proteins, Rats, Rats, Long-Evans, Serine, Signal Transduction, Transfection, bcl-X Protein, beta-Galactosidase