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Planarians are an accessible model system to study animal regeneration and stem cells. Over the last two decades, new molecular techniques have provided us with powerful tools to understand whole-body regeneration and pluripotent adult stem cells specifically. We describe a method for performing Chromatin Immunoprecipitation followed by sequencing (ChIP-seq) on planarian cells that relies on FACS to isolate different cell populations followed by immunoprecipitation and library preparation for next-generation sequencing. Whole-genome profiling of histone modifications enables a greater understanding of epigenetic mechanisms in development, pluripotency, and differentiation. This protocol adds to the growing list of functional genomic approaches to study whole-body regeneration in animals.

Original publication

DOI

10.1007/978-1-0716-2172-1_28

Type

Journal article

Journal

Methods Mol Biol

Publication Date

2022

Volume

2450

Pages

529 - 547

Keywords

Chromatin, Epigenetic regulation, FACS, Histone modifications, Immunoprecipitation, Planarian, Pluripotency, Animals, Chromatin, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation Sequencing, High-Throughput Nucleotide Sequencing, Planarians