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This work examines the separation of glucose oxidase (GOx) and catalase by ultrafiltration using 300 kDa MWCO polyethersulfone membranes. The effects of solution pH and ionic strength on the transmission of a single protein (either GOx or catalase) were quantified using the pulsed sample injection technique. Other effects including stirring speed and permeate flux on separation of the binary protein mixtures were also studied using parameter scanning ultrafiltration. Under optimized conditions, glucose oxidase transmission could reach more than 47% and the purity obtained in the permeate was greater than 93% after one stage ultrafiltration process even with higher molar ratio of catalase in the feed. © 2007 Elsevier B.V. All rights reserved.

Original publication




Journal article


Journal of Membrane Science

Publication Date





222 - 228