Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

The optimization of operating conditions for glucose oxidation to gluconic acid was systemically studied using an ultra-scaled down membrane bioreactor based on the parameter scanning ultrafiltration apparatus. The bioconversion of glucose to gluconic acid was carried out with freely suspended glucose oxidase (EC 1.1.3.4) as catalyst and dissolved oxygen saturated with air as oxygen supply. The bioreactor used PES ultrafiltration membrane with a MWCO of 70 kDa, which proved to retain glucose oxidase completely. The effects of pH, temperature, salt concentration, present of catalase, substrate-to-enzyme ratio, permeate flux and stirring speed on glucose conversion were examined. The results show that the addition of sodium chloride or catalase had a negative effect on glucose conversion. Under the most suitable conditions, a conversion of about 53.8% could be attained using the experimental apparatus. The identified operational and physicochemical conditions can guide the design and operation of enzymatic membrane bioreactor for partial oxidation of glucose to gluconic acid. © 2007 Elsevier B.V. All rights reserved.

Original publication

DOI

10.1016/j.memsci.2007.06.044

Type

Journal article

Journal

Journal of Membrane Science

Publication Date

15/09/2007

Volume

302

Pages

180 - 187