Cookies on this website

We use cookies to ensure that we give you the best experience on our website. If you click 'Accept all cookies' we'll assume that you are happy to receive all cookies and you won't see this message again. If you click 'Reject all non-essential cookies' only necessary cookies providing core functionality such as security, network management, and accessibility will be enabled. Click 'Find out more' for information on how to change your cookie settings.

Two systems utilizing extracts derived from nuclei of adenovirus-infected cells which synthesize adenovirus DNA in vitro were analysed for indications of ADP-ribosylation of virus proteins. On incubation with [32P]NAD or [14C]NAD, modification of the adenovirus T antigen could be demonstrated in one of these systems. ADP-ribosylation of adenovirus core proteins V and VII could also be demonstrated with both nuclear extracts. However, using 3-aminobenzamide, a specific inhibitor of poly(ADP-ribose) polymerase, there was no evidence either in vivo or in vitro that ADP-ribosylation played a critical role in the replication process.

Original publication

DOI

10.1099/0022-1317-64-2-477

Type

Journal article

Journal

J Gen Virol

Publication Date

02/1983

Volume

64 (Pt 2)

Pages

477 - 483

Keywords

Adenosine Diphosphate Ribose, Adenoviridae, Antigens, Viral, Antigens, Viral, Tumor, Benzamides, DNA Replication, DNA, Viral, HeLa Cells, Humans, In Vitro Techniques, Nucleoside Diphosphate Sugars, Viral Core Proteins, Viral Proteins, Virus Replication