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© 2019 Elsevier B.V. All rights reserved. Cryopreservation plays a significant role in tissue and organ banking. With the rapid development of tissue engineering, cryopreservation of tissue-engineered products is of great importance to meet the ‘ready-to-use’ requirement in clinical application and regenerative medicine. Cryopreservation can be achieved by conventional freezing and vitrification methods. Due to complexity and intrinsic differences between isolated cells and tissues, evaluation of the success of cryopreservation for tissues and organs is quite different from that of cells and more difficult. An overview of the methodology of cryopreservation is presented. Current development in cryopreservation of natural and engineered tissue has been reviewed as well.

Original publication

DOI

10.1016/B978-0-444-64046-8.00277-9

Type

Chapter

Book title

Comprehensive Biotechnology

Publication Date

01/01/2019

Pages

689 - 708