Interleukin-3 and interleukin-1α allow earlier bone marrow progenitors to respond to human colony-stimulating factor 1
Zhou YQ., Stanley ER., Clark SC., Hatzfeld JA., Levesque JP., Federici C., Watt SM., Hatzfeld A.
By using human bone marrow cells enriched for early progenitors by selective immunoadsorption and plated at low cell density (103 to 104 cells/mL/9.6 cm2) in semisolid methylcellulose culture, we have analyzed the cooperative effects of human colony-stimulating factor 1 (CSF-1), granulocyte-macrophage-CSF (GM-CSF), interleukin-1α (IL-1α), and gibbon as well as human recombinant IL-3 on the formation of monocytic colonies. CSF-1 alone stimulated mature monocytic colony formation by human CFU-M. However, in the presence of IL-3 and erythropoietin, CSF-1 stimulated maximal immature monocytic colony formation at low concentrations and inhibited the formation of granulomonocytic, erythrocytic, and mixed colonies. Cultures with CSF-1 and IL-3 contained more immature monocytic colonies than did cultures with CSF-1 alone. IL-1α alone had little effect. However, IL-1α in combination with optimal concentrations of either CSF-1, GM-CSF, or IL-3 increased the number of colonies containing immature or mature monocytic colonies.