Fractionation of proteins using ultrafiltration: Developments and challenges

In recent years ultrafiltratton has attracted significant interest as a potential technology for fractionating proteins. Although traditionally thought to be suitable for size-based separation with high-throughput but relatively low-resolution, recent research has demonstrated that it is possible to significantly increase the selectivity in protein fractionation using ultrafiltration while still maintaining its inherent high-throughput. This paper reviews recent developments in the area of protein fractionation using ultrafiltration, with focus on strategies employed to improve selectivity of separation. Several new techniques, technological developments, and their applications for selective ultrafiltration of proteins are also briefly introduced.